Polyclonal-Based ELISA for the Identification of Cyclohexanedione Analogs that Inhibit Maize Acetyl Coenzyme-A Carboxylase
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چکیده
منابع مشابه
Characterization of Maize Acetyl-Coenzyme A Carboxylase.
Maize (Zea mays L.) leaf acetyl-CoA carboxylase (ACCase) was purified about 500-fold by ammonium sulfate fractionation and gel filtration and blue Sepharose affinity and anion-exchange chromatography. Most ACCase activity (85%) recovered from the anion-exchange column was found in a highly purified fraction (specific activity 5.5 [mu]mol acid-stable product min-1 mg-1) that consisted primarily ...
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A partially dominant mutation exhibiting increased tolerance to cyclohexanedione and aryloxyphenoxypropionate herbicides was isolated by exposing susceptible maize (Zea mays) tissue cultures to increasingly inhibitory concentrations of sethoxydim (a cyclohexanedione). The selected tissue culture (S2) was greater than 40-fold more tolerant to sethoxydim and 20-fold more tolerant to haloxyfop (an...
متن کاملRat Mammary Acetyl Coenzyme A Carboxylase
The isolation of acetyl-CoA carboxylase from lactating rat mammary glands is described. A procedure was used which differs significantly from those employed by others who have isolated this enzyme from animal tissues. The purified enzyme catalyzes the carboxylation of 5.5 pmoles of acetylCoA per min per mg of protein under optimum assay conditions. Apparent K, values were determined for acetyl-...
متن کاملRegulation of Acetyl-Coenzyme A Carboxylase and Acetyl-Coenzyme A Synthetase in Spinach Chloroplasts
Acetyl-CoA Carboxylase, Acetyl-CoA Synthetase, Light Dependence o f Fatty Acid Synthesis in Chloroplasts In analogy to chloroplast fatty acid synthesis from acetate the key enzymes o f acetate fixation, acetyl-CoA synthetase and acetyl-CoA carboxylase, in rapidly Triton X-100 lysed spinach chloroplasts show an activation by light and deactivation in the dark. The stimulation o f acetyl-CoA carb...
متن کاملMolecular cloning of cDNA for acetyl-coenzyme A carboxylase.
Poly(A)+ RNA from lactating rat mammary glands was size-fractionated to enrich the relative amount of acetyl-CoA carboxylase mRNA. The enriched mRNA was used to generate a lambda gt11 cDNA library. Initial screening with polyclonal antiserum to acetyl-CoA carboxylase produced three positive clones. Western blot analysis revealed that two clones, lambda DH3 and lambda KH18, synthesized 165,000-d...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2001
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/84.1.143